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indexChallenge Topic:Which image data were used for this reconstruction?Start Frame (particle orientation)End Frame (particle orientation)Start Frame (final map)End Frame (final map)Did you use particle coordinates that were provided? (corrected)How many particle images were initially selected?Was 2D classification performed?Were other particle screening method(s) used?Was 3D classification performed?Was local drift corrected in particle images before map refinement?Was exposure weighting performed on particle images before map refinement?What was the approximate CPU usage at this stage (before refinement)?How many particle images were included at the start of map refinement?How many particle images were included in the final map?Was local drift in particle images corrected at this stage?Was exposure weighting performed at this stage?Map Refinement CPU UsageMap resolutionHow was the map resolution determined? (annotated)What was the temperature factor applied?Map Filtering (annotated)Mask submittedVoxel sizeWas any other type of map validation performed?
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101TRPVI Channelraw frames130130no267628yesnononono101982499624996yesyes273.3gold standard FSC, 0.143-45.647noneyes1.2no
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115TRPVI Channelraw frames130130no280849yesnoyesyesyes30003447034470yesyes10003.1gold standard FSC, 0.14338FSC-weightingyes1.2156no
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133TRPVI Channelraw frames316316yes (initial)35645nonononono03564535645nono25004gold standard FSC, 0.143109.587low passyes0.6078no
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135TRPVI Channelraw frames316316yes (initial)35645nonononono03564535645nono15003.8gold standard FSC, 0.143110.621low passyes0.6078yes
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146TRPVI Channelraw frames099099yes (initial)35645nonononono03564535645nono15004.1gold standard FSC, 0.1430Conversion to pseudoatomsno0.6078no
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156TRPVI Channelsummed particle stacksn/an/an/an/ayes (initial)35645yesnononono1002575725757nono50003.3gold standard FSC, 0.1430noneno1.2156no
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161TRPVI Channelsummed particle stacksn/an/an/an/ayes (initial)35645nonononono23564535645nono13.2gold standard FSC, 0.14380FSC-weightingyes1.2156yes
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163TRPVI Channelsummed particle stacksn/an/an/an/ayes (initial)35286yesyesnonono203528917280nono15003.7gold standard FSC, 0.1430FSC-weighting, normalizationyes1.22no