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indexChallenge Topic:Which image data were used for this reconstruction?Start Frame (particle orientation)End Frame (particle orientation)Start Frame (final map)End Frame (final map)Did you use particle coordinates that were provided? (corrected)How many particle images were initially selected?Was 2D classification performed?Were other particle screening method(s) used?Was 3D classification performed?Was local drift corrected in particle images before map refinement?Was exposure weighting performed on particle images before map refinement?What was the approximate CPU usage at this stage (before refinement)?How many particle images were included at the start of map refinement?How many particle images were included in the final map?Was local drift in particle images corrected at this stage?Was exposure weighting performed at this stage?Map Refinement CPU UsageMap resolutionHow was the map resolution determined? (annotated)What was the temperature factor applied?Map Filtering (annotated)Mask submittedVoxel sizeWas any other type of map validation performed?
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106beta-Galactosidaseraw frames125125no164460yesnoyesyesyes50009121191211yesyes10002.8gold standard FSC, 0.143-67FSC-weightingyes1.275yes
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113beta-Galactosidaseraw frames039039yes (initial)23541yesnoyesnoyes17201748517485nono15093.5gold standard FSC, 0.143-129noneno1.275no
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116beta-Galactosidaseraw frames038038no50139nonononono05013919465nono256003.4gold standard FSC, 0.1430Carbon cross section weightingyes0.64yes
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134beta-Galactosidaseraw frames099099yes (initial)22894yesnonoyesno6002289422894nono200003.3gold standard FSC, 0.1430Conversion to pseudoatomsno0.9058no
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138beta-Galactosidaseraw frames234234yes (initial)22824nonononono02282422824nono12003.3gold standard FSC, 0.143104.253low passyes0.6375yes
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139beta-Galactosidaseraw frames234234yes (initial)22824nonononono02282422824nono15003.3gold standard FSC, 0.14392.56low passyes0.6375yes
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154beta-Galactosidasesummed micrographsn/an/an/an/ano29210yesnononono1002841423285nono50003.1half set FSC, 0.990noneyes0.6375no
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157beta-Galactosidaseraw frames420414yes (initial)23541yesyesnonoyes50106269600noyes10003.4gold standard FSC, 0.1430FSC-weighting, normalizationyes1.02yes
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159beta-Galactosidaseraw frames099099yes (initial)22894yesnononono6002289422894nono200003.3gold standard FSC, 0.1430normalizationyes1.2no
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160beta-Galactosidaseraw frames020020yes (initial)21687yesyesnonono252002115881nono20003.5gold standard FSC, 0.1430FSC-weightingyes0.96yes
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164beta-Galactosidaseraw frames099099yes (initial)22894yesnononono6002289422894nono200003.3gold standard FSC, 0.1430normalizationyes1.2no
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167beta-Galactosidaseraw frames099099yes (initial)22894yesnononono6002289422894nono200003.3gold standard FSC, 0.1430normalizationyes1.2no