published Sun, 11/22/2015 - 15:54
Below are compiled questions/answers regarding the map challenge that may be of interest to all of the participants. We'll update this FAQ as needed.
Challenge Phase
1. Is the Cs listed in the target table (2.7) correct for the Apoferritin data? No. There was an error in the script to generate the metadata requested for the challenge. The manufacturer specified Cs value for the Polara is 2.0 mm, but this has never been measured accurately for the instrument. In practice, this is an somewhat arbitrary fitting parameter that can be input during analysis of the raw data or refined during fitting. Followup question: What Cs do the defocus values provided in the particle stack refer to? The provided defocus values assume Cs = 2.7. posted Nov 20, 2015, thanks to Niko Grigorieff for questions, and Chris Russo for answers